Ref ID:	27922
Ref Type:	Journal Article
Authors:	Castillo, Cristina Cobo Tanaka, Katsunori Sato, Yo-Ichiro Ishikawa, Ryuji Bellina, Bérénice Higham, Charles Chang, Nigel Mohanty, Rabi Kajale, Mukund Fuller, Dorian Q.
Title:	Archaeogenetic study of prehistoric rice remains from Thailand and India: evidence of early <i>japonica</i> in South and Southeast Asia
Date:	2016
Source:	Archaeological and Anthropological Sciences
DOI:	10.1007/s12520-015-0236-5
Abstract:	We report a successful extraction and sequencing of ancient DNA from carbonized rice grains (<i>Oryza sativa</i>) from six archaeological sites, including two from India and four from Thailand, ranging in age from ca. 2500 to 1500 BP. In total, 221 archaeological grains were processed by PCR amplification and primary-targeted fragments were sequenced for comparison with modern sequences generated from 112 modern rice populations, including crop and wild varieties. Our results include the genetic sequences from both the chloroplast and the nuclear genomes, based on four markers from the chloroplast and six from the nuclear genome. These markers allow differentiation of <i>indica</i> rice from <i>japonica</i> rice, the two major subspecies of Asian rice (<i>O. sativa</i>) considered to have separate geographical origins. One nuclear marker differentiates tropical and temperate forms of subspecies <i>japonica</i>. Other markers relate to phenotypic variation selected for under domestication, such as non-shattering, grain stickiness (waxy starch) and pericarp colour. Recovery and identification of sequences from nuclear markers was generally poor, whereas recovery of chloroplast sequences was successful, with at least one of four markers recovered in 61 % of archaeological grains. This allowed for successful differentiation of <i>indica</i> or <i>japonica</i> subspecies variety, with <i>japonica</i> identified in all the Thai material and a mixture of <i>indica</i> and <i>japonica</i> chloroplasts in the two Indian assemblages. Rice subspecies was also assessed through conventional archaeobotanical methods relying on grain metrics, based on measurements from 13 modern populations and 499 archaeological grains. Grain metrics also suggest a predominance of <i>japonica</i>-type grains in the Southeast Asian sites and a mixture of <i>japonica</i> and <i>indica</i> in the Indian sites with <i>indica</i> in the minority. The similar results of grain metrics and ancient DNA (aDNA) affirm that grain measurements have some degree of reliability in rice subspecies identification. The study also highlights the great potential of ancient DNA recovery from archaeological rice. The data generated in the present study adds support to the model of rice evolution that includes hybridization between <i>japonica</i> and proto-<i>indica</i>.
Date Created:	5/26/2015
Volume:	8
Number:	3
Page Start:	523
Page End:	543