Ref ID:	34740
Ref Type:	Journal Article
Authors:	DeNiro, Michael J. Epstein, Samuel
Title:	Mechanism of carbon isotope fractionation associated with lipid synthesis
Date:	1977
Source:	Science
Abstract:	The low 13C/12C ratio of lipids is shown to result from isotopic fractionation during the oxidation of pyruvate to acetyl coenzyme A. In vitro analysis of the kinetic isotope effects of this reaction indicates that there will be a large temperature-dependent difference in the 13C/12C ratio between the methyl and carbonyl carbon atoms of acetyl coenzyme A and between the those carbon atoms of lipid components which derive from them. Provides a point-by-point analysis of the chemical processes involved in the breakdown of 13C. In sum, carbohydrates would be a formation of \ide novo\i synthesis and a assimilation of precursors from diet (pg 263).
Date Created:	7/5/2001
Volume:	197
Number:	4300
Page Start:	261
Page End:	263